Effect of successive milt collection on sperm quality and reproduction of wild and cultured endangered caspian brown trout, Salmo trutta

In the present study, the effect of successive milt collection was investigated on spermatological parameters and reproduction successes of wild and cultured endangered caspian brown trout. The milt collection was done 3 times with two weeks intervals. After milt collection, amount of milt was allocated for milt quality evaluation and reminders used for fertilization. According to results, in wild fish, the values of fertilization rate, eyeing rate, hatching rate, milt volume and duration of sperm motility decreased during successive stripping (P0.05). Also, the percentage of abnormal spermatozoa increased during successive stripping in wild fish (P0.05). In cultured fish, similar results were observed about fertilization rate, milt volume and duration of sperm motility (P0.05). Also, the survival rates of larvae were statistically different between second and third stripping (P